Fig. 1
From: Metabolic engineering of energycane to hyperaccumulate lipids in vegetative biomass
Multi-gene expression and RNAi suppression constructs that were cotransformed into energycane. A The overexpression cassette of ZmDGAT1-2 and SiCysOLE1. B RNAi hairpin cassette of TGD1 and SDP1. Neomycin phosphotransferase II (NPTII) is used as the selectable marker. ZmDGAT1-2: Diacylglycerol acyltransferase1-2 from Zea mays. SiCysOLE1: Cysteine oleosin1 from Sesamum indicum. TGD1: Trigalactosyl diacylglycerol1 from Saccharum spp. hybrid. SDP1: Sugar-dependent1 from Saccharum spp. hybrid. pZmUbi and pSbUbi: Ubiquitin promoters from Zea mays and Sorghum bicolor, respectively. tNos: Nos terminator from Agrobacterium tumefaciens. tSbHSP: The heat shock protein 18 terminator from Sorghum bicolor. p35S and t35S: 35S promoter and terminator, respectively. Half arrows indicate primers used to detect transgenes