Table 1 Half maximal effective concentration (EC50) and limit of detection (LOD) values of antibodies that were determined from the titration curves of indirect ELISA. n.d. = not detected
From: Generation of a recombinant antibody for sensitive detection of Pseudomonas aeruginosa
P. aeruginosa strain | Antibody | EC50 (CFU/mL) | LOD (CFU/mL) |
|---|---|---|---|
UCBPP-PA14 | Polyclonal antibody (pAb) | 3.08 ± 0.31 × 107 | 2.92 × 106 |
UCBPP-PA14 | Monoclonal antibody (mAb) | 7.46 ± 3.27 × 106 | 4.69 × 106 |
UCBPP-PA14 | Recombinant antibody (rAb) | n.d | n.d |
ATCC 27,853 | pAb | 5.03 ± 0.98 × 104 | 3.50 × 102 |
ATCC 27,853 | mAb | 2.77 ± 0.37 × 106 | 4.01 × 105 |
ATCC 27,853 | rAb | 1.01 ± 0.28 × 106 | 2.43 × 105 |
ATCC BAA-2108 | pAb | 1.30 ± 0.08 × 105 | 4.42 × 105 |
ATCC BAA-2108 | mAb | 2.15 ± 0.27 × 106 | 1.37 × 105 |
ATCC BAA-2108 | rAb | 2.91 ± 0.30 × 106 | 7.62 × 105 |