Generation of genome-edited dogs by somatic cell nuclear transfer

BMC Biotechnology

Table 2 In situ design of potential off-target locus

	Sequences	Chr	Position	Dir	Mismatch	Mutation (#1/#2)
On-target	GTAGATGTCATGAGACGAGCNGG	5	61577522	+	0
Off-target 1	GTAGATGTgATGAcAgGAGCTGG	8	12929143	+	3	WT/WT
Off-target 2	cTAaATGTCATGAGAaGAGCAGG	30	14653215	+	3	WT/WT
Off-target 3	GTAGATGTCAgGAGAgGAGgGGG	7	39969232	+	3	WT/WT
Off-target 4	GTgGATGgCATGAGACGAGtGGG	17	53221459	−	3	WT/WT
Off-target 5	GTAGATGTgATGAaAtGAGCTGG	11	51558749	+	3	WT/WT
Off-target 6	aTAGATGTCATGAGAtGAGCTGG	24	32851089	−	2	WT/WT

Letters with lowercase indicate mismatches with on-target sequence
Chr chromosome number, Dir direction, Mismatch number of mismatches, Mutation sequence compared with WT

ISSN: 1472-6750