Fig. 1
From: Generation of genome-edited dogs by somatic cell nuclear transfer
Design and expression of CRISPR-Cas9 vector. A Guide RNA (gRNA, red letters) sequence selected from exon 2 of canine DJ-1 (highlighted in yellow). The bold, underlined letters at the front and end of the sequences were primer sets used for sequencing analysis. B Schemes for CRISPR-Cas9 vector construct used in the study. Expression of the vector in transfected cells was confirmed based on EGFP expression evaluated using C bright view microscopy and D ultraviolet light