Fig. 4
From: αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8
Delivery of Gaussia Luciferase (GL) into sEVs by fusion expression. HEK293F cells were transfected with pCDNA3.4/MFG-E8-GL and pCDNA3.4/CD9-GL respectively and sEVs were isolated. Both M8-GL (a and Additional file 1: Figures S9, S11) and CD9-GL (b and Additional file 1: Figures S10, S11) were found in the sEVs by western blotting analysis. The relative GL concentration in sEVs was analyzed by comparing M8-GL/CD63 and CD9-GL/CD63 (c). And the luciferase activity in two sEVs was compared by analyzing the catalytic activity of the enzyme (d)