Table 5 Details of the 1-cell or 2-cells targeting rate for each individual project
Gene name | Procedure | Number of pups born | Number of pups with a loxP site targeted at the 1-cell stage | Targeting frequency at the 1-cell stage (%)* | Number of pups with a loxP sites targeted at the 2-cells stage | Targeting frequency at the 2-cells stage (%)* | Deletion between exon(s)** |
|---|---|---|---|---|---|---|---|
Icam1 | Consecutive sequential electroporation | 24 | 7 | 29 | 6 | 25 | NA |
Lox | Consecutive sequential electroporation | 22 | 3 | 14 | 10 | 45 | NA |
Sar1b | Consecutive sequential electroporation | 8 | 3 | 38 | 2 | 25 | NA |
Loxl1 | Non-consecutive sequential electroporation | 26 | 1 | 4 | 5 | 19 | 3 |
Pard6a | Non-consecutive sequential electroporation | 9 | 3 | 33 | 0 | 0 | 1 |
Pard6g | Non-consecutive sequential electroporation | 26 | 9 | 35 | 1 | 4 | NA |
Clcf1 | Non-consecutive sequential electroporation | 8 | 3 | 38 | 0 | 0 | NA |
Mapkapk5 | Non-consecutive sequential electroporation | 17 | 2 | 12 | 2 | 12 | 4 |