Table 4 Details of the projects successfully completed using the non-consecutive sequential electroporation procedure
Gene name | Procedure | loxP site inserted | Number of embryos electroporated | Number of embryos implanted | Number of surgeries | Number of gestations | Number of pups born | Percentage of live born animal (%)* | Number of pups properly targeted | Targeting efficiency (%)** |
|---|---|---|---|---|---|---|---|---|---|---|
Loxl1 | Non-consecutive sequential electroporation | 1st loxP site | 393 | 305 | 13 | 10 | 26 | 9 | 2 | 8 |
2nd loxP site (targeting strand) | 264 | 227 | 9 | 6 | 34 | 15 | 1 | 3 | ||
2nd loxP site (non-targeting strand) | 136 | 132 | 6 | 3 | 17 | 13 | 1 | 6 | ||
Pard6a | Non-consecutive sequential electroporation | 1st loxP site | 210 | 107 | 4 | 4 | 9 | 8 | 1 | 11 |
2nd loxP site | 147 | 114 | 5 | 5 | 30 | 26 | 4 | 13 | ||
Pard6g | Non-consecutive sequential electroporation | 1st loxP site | 212 | 173 | 7 | 7 | 26 | 15 | 2 | 8 |
2nd loxP site | 180 | 146 | 6 | 4 | 21 | 14 | 5 | 24 | ||
Clcf1 | Non-consecutive sequential electroporation | 1st loxP site | 102 | 80 | 3 | 2 | 8 | 10 | 2 | 25 |
2nd loxP site | 243 | 196 | 7 | 3 | 24 | 12 | 3 | 13 | ||
Mapkapk5 | Non-consecutive sequential electroporation | 1st loxP site | 165 | 138 | 6 | 5 | 17 | 12 | 1 | 6 |
2nd loxP site | 159 | 145 | 7 | 6 | 36 | 25 | 3 | 8 |