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Table 2 Details of the results obtained for the Icam1 project using two different reagent concentrations

From: Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci

Gene name

Procedure

Reagent concentration (per session, μM)

Number of embryos electroporated

Number of embryos implanted

Number of surgeries

Number of gestations

Number of pups born

Percentage of live born animals (%)*

Icam1

Consecutive sequential electroporation

4:4:10

100

86

4

3

3

3

Icam1

Consecutive sequential electroporation

2:2:5

100

81

4

4

18

22

  1. *Calculated by dividing the number of pups born by the number of implanted embryos