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Table 1 Details of the conditional allele targeting projects

From: Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci

Gene name

Strain

Targeted exons

Distance between both loxP sites (in base pairs)

Limitations

Plan used for project completion

Icam1

C57BL/6J

4 to 7

2926

Distance between both loxP sites**

Strategy A

Lox

C57BL/6J

2

522

Sequence complexity***

Strategy A

Sar1b

C57BL/6N

2

608

None

Strategy A

Loxl1

C57BL/6J

2

617

None

Strategy B

Pard6a

C57BL/6J

2

543

None

Strategy B

Pard6g*

C57BL/6J

1

1463–1492

Sequence complexity***

Strategy B

Clcf1

C57BL/6N

3

2509

Distance between both loxP sites**

Strategy B

Mapkapk5

C57BL/6J

6

1000

None

Strategy B

  1. *Pups properly targeted with loxP sites in the up position from two different guides
  2. **Megamer, IDT
  3. ***Megamer could not be synthesized