Skip to main content
Fig. 2 | BMC Biotechnology

Fig. 2

From: Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci

Fig. 2

Consecutive sequential electroporation was successfully applied to generate Icam1 floxed animals. Schematic representation highlighting the primer positions and genotyping strategy used for characterization of Icam1 floxed F0 animals. PCR results from primers 2–5 combination are depicted in the upper middle panel. PCR results and sequencing alignment from primers 1–5 combination are depicted in the lower left panel. PCR results and sequencing alignment from primers 2–6 combination are depicted in the lower right panel. Primers used for sequencing are highlighted in red. The same strategy was applied to complete a total of three different projects (Icam1, Lox, Sar1b)

Back to article page