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Table 3 Comparison of anti-viral activity for different concentrations of IFNα and SGLuc-IFNα

From: Method for quantification of porcine type I interferon activity using luminescence, by direct and indirect means

 

Source

Dilution

Concentration

PFU (± SD)

pg/mL (± SD)

IFNα

Batch 1

1/102400

404 ± 151

80 ± 9.0

IFNα

Batch 1

1/51200

807 ± 301

33 ± 3.2

SGLuc-IFNα

Batch 1

1/12800

738 ± 114

27 ± 9.6

SGLuc-IFNα

Batch 2

1/12800

948 ± 114

5.0 ± 1.7

SGLuc-IFNα

Batch 3

1/12800

1449 ± 214

3.7 ± 1.5

  1. Concentration, as determined by ELISA, and SVV plaque forming units (PFU) in triplicate wells from select dilutions of IFNα and SGLuc-FNα. Three preparations of SGLuc-IFNα were diluted to 1/12,800 for comparison across batches