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Table 3 Comparison of anti-viral activity for different concentrations of IFNα and SGLuc-IFNα

From: Method for quantification of porcine type I interferon activity using luminescence, by direct and indirect means

  Source Dilution Concentration PFU (± SD)
pg/mL (± SD)
IFNα Batch 1 1/102400 404 ± 151 80 ± 9.0
IFNα Batch 1 1/51200 807 ± 301 33 ± 3.2
SGLuc-IFNα Batch 1 1/12800 738 ± 114 27 ± 9.6
SGLuc-IFNα Batch 2 1/12800 948 ± 114 5.0 ± 1.7
SGLuc-IFNα Batch 3 1/12800 1449 ± 214 3.7 ± 1.5
  1. Concentration, as determined by ELISA, and SVV plaque forming units (PFU) in triplicate wells from select dilutions of IFNα and SGLuc-FNα. Three preparations of SGLuc-IFNα were diluted to 1/12,800 for comparison across batches