Fig. 4

A Luciferase readings for serial dilutions of SGLuc-IFNα and β samples show strong linear correlation over tested dilutions of 1/50 to 1/6400. B Luciferase activity, N = 7 replicates per sample, in media produced from three separate transfections of SGLuc-IFNα expressing plasmid. Corresponding mean plaque numbers for each sample are within bars with standard deviation in parenthesis below; N = 3 replicates per sample. RLUs/0.5 s, relative luciferase units per half second. C Triplicate wells for plaque assays evaluating SGLuc-IFNα efficacy against FMDV. Tested dilutions demonstrate a reduction in plaque numbers from untreated control values of 200 to an average of 14.7 and 29.3 for dilutions of 1:1600 and 1:3200, respectively