Isolation and identification of a feather degrading Bacillus tropicus strain Gxun-17 from marine environment and its enzyme characteristics

Table 4 Effect of various chemical agents on the keratinase

Chemical reagent	Concentration (mM)	Residual activity (%)	Chemical reagent	Concentration (%; v/v)	Residual activity (%)
None	–	100.00 ± 1.25^d
EDTA	2.5	98.57 ± 0.55^d	Isopropanol	2.5	32.85 ± 2.04^e
	5	97.15 ± 1.14^d		5	13.91 ± 6.62^f
SDS	2.5	98.23 ± 1.61^d	DMSO	2.5	115.00 ± 4.55^c
	5	94.30 ± 2.23^d		5	100.61 ± 3.45^d
PMSF	2.5	-3.47 ± 1.53^g	Mercaptoethanol	2.5	395.44 ± 11.56^b
	5	4.81 ± 3.41^fg		5	444.67 ± 0.78^a

Superscript letter(s) a, b, c, d, e, f and g down the column were used to indicate significant difference at P < 0.05

ISSN: 1472-6750