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Table 4 Effect of various chemical agents on the keratinase

From: Isolation and identification of a feather degrading Bacillus tropicus strain Gxun-17 from marine environment and its enzyme characteristics

Chemical reagent Concentration (mM) Residual activity (%) Chemical reagent Concentration (%; v/v) Residual activity (%)
None 100.00 ± 1.25d    
EDTA 2.5 98.57 ± 0.55d Isopropanol 2.5 32.85 ± 2.04e
  5 97.15 ± 1.14d   5 13.91 ± 6.62f
SDS 2.5 98.23 ± 1.61d DMSO 2.5 115.00 ± 4.55c
  5 94.30 ± 2.23d   5 100.61 ± 3.45d
PMSF 2.5 -3.47 ± 1.53 g Mercaptoethanol 2.5 395.44 ± 11.56b
  5 4.81 ± 3.41 fg   5 444.67 ± 0.78a
  1. Superscript letter(s) a, b, c, d, e, f and g down the column were used to indicate significant difference at P < 0.05