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Table 4 Effect of various chemical agents on the keratinase

From: Isolation and identification of a feather degrading Bacillus tropicus strain Gxun-17 from marine environment and its enzyme characteristics

Chemical reagent

Concentration (mM)

Residual activity (%)

Chemical reagent

Concentration (%; v/v)

Residual activity (%)

None

–

100.00 ± 1.25d

   

EDTA

2.5

98.57 ± 0.55d

Isopropanol

2.5

32.85 ± 2.04e

 

5

97.15 ± 1.14d

 

5

13.91 ± 6.62f

SDS

2.5

98.23 ± 1.61d

DMSO

2.5

115.00 ± 4.55c

 

5

94.30 ± 2.23d

 

5

100.61 ± 3.45d

PMSF

2.5

-3.47 ± 1.53 g

Mercaptoethanol

2.5

395.44 ± 11.56b

 

5

4.81 ± 3.41 fg

 

5

444.67 ± 0.78a

  1. Superscript letter(s) a, b, c, d, e, f and g down the column were used to indicate significant difference at P < 0.05