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Table 3 Optimization of fermentation conditions using orthogonal test

From: Isolation and identification of a feather degrading Bacillus tropicus strain Gxun-17 from marine environment and its enzyme characteristics

Test no Factor Keratinase activity (U/mL)
A B C D
1 1 1 1 1 74.81 ± 2.69b
2 1 2 2 2 73.20 ± 5.56b
3 1 3 3 3 79.93 ± 5.31b
4 2 1 2 3 19.76 ± 4.84c
5 2 2 3 1 3.81 ± 3.64d
6 2 3 1 2 79.23 ± 7.38b
7 3 1 3 2 107.82 ± 4.63a
8 3 2 1 3 102.30 ± 3.50a
9 3 3 2 1 71.81 ± 1.15b
k1 75.98 67.46 85.45 50.14  
k2 34.27 59.77 54.92 86.75  
k3 93.98 76.99 63.85 67.33  
R 59.71 17.22 21.59 36.61  
The impact of factors A > D > C > B     
Optimization level A3 B3 C1 D2  
  1. Superscript letter(s) a, b, c and d down the column were used to indicate significant difference at P < 0.05