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Table 3 Optimization of fermentation conditions using orthogonal test

From: Isolation and identification of a feather degrading Bacillus tropicus strain Gxun-17 from marine environment and its enzyme characteristics

Test no

Factor

Keratinase activity (U/mL)

A

B

C

D

1

1

1

1

1

74.81 ± 2.69b

2

1

2

2

2

73.20 ± 5.56b

3

1

3

3

3

79.93 ± 5.31b

4

2

1

2

3

19.76 ± 4.84c

5

2

2

3

1

3.81 ± 3.64d

6

2

3

1

2

79.23 ± 7.38b

7

3

1

3

2

107.82 ± 4.63a

8

3

2

1

3

102.30 ± 3.50a

9

3

3

2

1

71.81 ± 1.15b

k1

75.98

67.46

85.45

50.14

 

k2

34.27

59.77

54.92

86.75

 

k3

93.98

76.99

63.85

67.33

 

R

59.71

17.22

21.59

36.61

 

The impact of factors

A > D > C > B

    

Optimization level

A3

B3

C1

D2

 
  1. Superscript letter(s) a, b, c and d down the column were used to indicate significant difference at P < 0.05
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BMC Biotechnology

ISSN: 1472-6750

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