From: A simplified Gibson assembly method for site directed mutagenesis by re-use of standard, and entirely complementary, mutagenesis primers
Component
Step I
Step III
2X KAPA HiFi HotStart ReadyMix
12.5 µl
Template
1 µl (10 ng)
1 µl (Gibson mix)
SDM_Primer_F (10 µM stock)
0.75 µl
SDM_Primer_R (10 µM stock)
ddH2O
Up to 25 µl