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Table 2 Reaction mixture for the KAPA HiFi HotStart ReadyMix PCR Kit

From: A simplified Gibson assembly method for site directed mutagenesis by re-use of standard, and entirely complementary, mutagenesis primers

Component

Step I

Step III

2X KAPA HiFi HotStart ReadyMix

12.5 µl

12.5 µl

Template

1 µl (10 ng)

1 µl (Gibson mix)

SDM_Primer_F (10 µM stock)

0.75 µl

0.75 µl

SDM_Primer_R (10 µM stock)

0.75 µl

0.75 µl

ddH2O

Up to 25 µl

Up to 25 µl