An efficient and specific CRISPR-Cas9 genome editing system targeting soybean phytoene desaturase genes

BMC Biotechnology

Table 3 Summary of phenotypes and CRISPR/Cas9-induced mutations in GmPDS8 T1 line #5 plants

Plant	Phenotypes	Transgene detection	Indel frequency in GmPDS18g (%)	Indel frequency in GmPDS11g (%)
#5–1	Dwarf with albino leaves	+	100	93
#5–2	Dwarf with albino leaves	+	100	100
#5–3	Non-dwarf, with green leaves	−	0	0
#5–4	Dwarf with albino leaves	+	100	100
#5–5	Non-dwarf, with green leaves	−	31	10
#5–6	Non-dwarf, with green leaves	−	40	100

Indel frequency (%) is calculated as number of clones showing mutations divided by total number of sequenced clones

ISSN: 1472-6750