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Table 2 Summary of CRISPR/Cas9-induced mutations in T0 plants

From: An efficient and specific CRISPR-Cas9 genome editing system targeting soybean phytoene desaturase genes

Construct

# of sequenced plants

# of plants verified for transformation

# of plants with mutations in GmPDS18g

# of plants with mutations in GmPDS11g

Mutation efficiency (%)

Indel frequency (%)

GmPDS1

2

2

2

0

100

6.5–16

GmPDS3

8

5

5

0

100

8–88

GmPDS7

4

4

0

3

75

9–21

GmPDS8

11

10

9

9

90

3–100

GmPDS9

6

5

4

5

90

4–100

  1. Mutation efficiency (%) is calculated as number of plants showing mutations divided by number of plants verified for transformation
  2. Indel frequency (%) is calculated as number of clones showing mutations divided by total number of sequenced clones
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BMC Biotechnology

ISSN: 1472-6750

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