Fig. 7

Detection of mutations in GmPDS8 T1 progenies derived from T0 plant #5. A Representative sequencing chromatograms showing mutations. The 20-nt target sequence is highlighted in blue, PAM sequence is indicated in the green box, and mutations are circled in red. B Representative mutations at target sites on chromosome 18 and chromosome 11. Individual T1 plant harboring the mutation is indicated to the right of each sequence. Wild type sequence is shown at the top with the 20-nt guide sequence in blue and PAM sequence in green. Mutations are shown in red. The number of mutated nucleotides is indicated to the right of each sequence. -: deletion, + : insertion, S: substitution. The number of clones for each mutation is indicated in brackets