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Fig. 3 | BMC Biotechnology

Fig. 3

From: An efficient and specific CRISPR-Cas9 genome editing system targeting soybean phytoene desaturase genes

Fig. 3

Detection of mutations in GmPDS1, GmPDS3, and GmPDS7 T0 plants. A Examples of sequencing chromatograms showing mutations. The 20-nt target sequence is highlighted in blue, PAM sequence is indicated in the green box, and mutations are circled in red. B Representative mutations at target sites. Individual T0 plant harboring the mutation is indicated to the right of each sequence. Wild type sequence is shown at the top with the 20-nt guide sequence in blue and PAM sequence in green. Mutations are shown in red. The number of mutated nucleotides is indicated to the right of each sequence. -: deletion, + : insertion. The number of clones for each mutation is indicated in brackets

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BMC Biotechnology

ISSN: 1472-6750

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