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Table 2 ANOVA table of screening experiment narrating factors’ significance on SHuffle T7 growth

From: Media optimization for SHuffle T7 Escherichia coli expressing SUMO-Lispro proinsulin by response surface methodology

Source

Degree of freedom

Adjusted sums of squares

Adjusted mean squares

F value

p value

Model

9

3.35

0.37

14.67

0.0

Linear

8

2.95

0.37

14.5

0.0

N source type

1

0.0

0.0

0.01

0.935

N source conc. (%)

1

0.18

0.18

7.1

0.024

Yeast extract conc. (%)

1

0.56

0.56

22.12

0.001

pH

1

1.57

1.57

61.95

0.0

Phosphate buffer

1

0.12

0.12

4.96

0.05

NaCl conc. (mM)

1

0.0

0.0

0.00

0.989

KCl conc. (mM)

1

0.14

0.14

5.38

0.043

MgCl2 conc. (mM)

1

0.37

0.37

14.48

0.003

Curvature

1

0.41

0.41

16.07

0.002

Error

10

0.25

0.02

  

Lack-of-fit

6

0.16

0.03

1.07

0.498

Pure error

4

0.1

0.02

  

Total

19

3.61