Table 1 Generated experimental runs for factor screening via PBD and corresponding responses
Run | N Type | N (1–4%) | Yeast extract (1–4%) | pH (6–8) | Buffer | NaCl (0-17 mM) | KCl (0-10 mM) | MgCl2 (5-15 mM) | MgSO4 (5-15 mM) | Glycerol (0–5%) | Glucose (0-10 mM) | CDW (g/L) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | Peptone | 2.5 | 2.5 | 7 | + | 8.5 | 5 | 10 | 10 | 2.5 | 10 | 2.18 |
2 | Tryptone | 4 | 4 | 8 | + | 17 | 10 | 5 | 15 | 0 | 0 | 2.15 |
3 | Peptone | 4 | 1 | 8 | − | 0 | 10 | 5 | 5 | 0 | 20 | 2.1 |
4 | Peptone | 2.5 | 2.5 | 7 | − | 8.5 | 5 | 10 | 10 | 2.5 | 10 | 2.04 |
5 | Tryptone | 1 | 1 | 8 | − | 17 | 0 | 15 | 15 | 0 | 20 | 1.98 |
6 | Peptone | 1 | 4 | 8 | + | 17 | 0 | 5 | 5 | 5 | 20 | 1.95 |
7 | Peptone | 4 | 1 | 8 | + | 0 | 0 | 15 | 15 | 5 | 0 | 1.95 |
8 | Peptone | 2.5 | 2.5 | 7 | − | 8.5 | 5 | 10 | 10 | 2.5 | 10 | 1.87 |
9 | Tryptone | 2.5 | 2.5 | 7 | + | 8.5 | 5 | 10 | 10 | 2.5 | 10 | 1.87 |
10 | Tryptone | 2.5 | 2.5 | 7 | + | 8.5 | 5 | 10 | 10 | 2.5 | 10 | 1.84 |
11 | Tryptone | 2.5 | 2.5 | 7 | − | 8.5 | 5 | 10 | 10 | 2.5 | 10 | 1.82 |
12 | Peptone | 1 | 1 | 6 | − | 17 | 10 | 5 | 15 | 5 | 0 | 1.8 |
13 | Tryptone | 2.5 | 2.5 | 7 | − | 8.5 | 5 | 10 | 10 | 2.5 | 10 | 1.8 |
14 | Peptone | 2.5 | 2.5 | 7 | + | 8.5 | 5 | 10 | 10 | 2.5 | 10 | 1.78 |
15 | Tryptone | 1 | 1 | 6 | + | 0 | 0 | 5 | 5 | 0 | 0 | 1.77 |
16 | Tryptone | 1 | 4 | 8 | − | 0 | 10 | 15 | 5 | 5 | 0 | 1.69 |
17 | Tryptone | 4 | 1 | 6 | + | 17 | 10 | 15 | 5 | 5 | 20 | 1.35 |
18 | Peptone | 1 | 4 | 6 | + | 0 | 10 | 15 | 15 | 0 | 20 | 1.2 |
19 | Tryptone | 4 | 4 | 6 | − | 0 | 0 | 5 | 15 | 5 | 20 | 0.94 |
20 | Peptone | 4 | 4 | 6 | − | 17 | 0 | 15 | 5 | 0 | 0 | 0.42 |