Fig. 8From: Media optimization for SHuffle T7 Escherichia coli expressing SUMO-Lispro proinsulin by response surface methodologyPOI soluble expression and Purification. Coomassie stained 12% SDS-PAGE: A POI soluble expression in LB and OM-I media. M. Protein Marker. 1–3: POI soluble expression in LB media. 4–6: POI soluble expression in OM-I media. B SU-INS POI IMAC purification. M. Protein Ladder. 1: Cell lysate supernatant (Unpurified), 2: Purified POIBack to article page