Fig. 3
From: Enzymatic degradation is an effective means to reduce aflatoxin contamination in maize
Detection of aflatoxin-degrading enzyme in maize kernel proteome by mass spectroscopy. Shown is the complete amino acid sequence of the inserted ER-targeted aflatoxin-degrading enzyme protein. Underlined is both the N-terminal ER signal peptide that is expected to be cleaved in the mature protein and the C-terminal ER-retention tag. Highlighted are the peptides detected in total soluble protein extracted from developing Enz maize kernels. This represents a 40% detection coverage and the inserted aflatoxin-degrading protein was detected in all three Enz transgenic lines assayed