Fig. 2
From: Enzymatic degradation is an effective means to reduce aflatoxin contamination in maize
Expression of aflatoxin-degrading enzyme cassette in maize kernels. Expression of the Enz cassette was assessed using total RNA extracted from approximately 10 DAP developing kernels. A RT-PCR amplification using primers specific to the Enz open reading frame. The expected amplicon of 743Â bp was detected in the 3 lines tested, Enz lines 7, 8, 10 and absent in a nontransgenic Null control. gDNA is genomic DNA isolated from transgenic Enz line 10. B RT-PCR amplification of a control endogenous gene actin using actin-specific primers that are adjacent to an intron. The expected cDNA 157Â bp amplicon is detected in Enz transgenic lines 7, 8, 10 and a Null nontransgenic control. Genomic DNA (gDNA) amplicon actin expected size of 264Â bp was not detected in the 3 Enz cDNA samples, indicating these samples do not contain any contaminating gDNA. M denotes DNA size marker