Fig. 4From: Expression, purification and characterisation of a human anti-CDK4 single-chain variable fragment antibodyDetermination of affinity of AK2 by noncompetitive ELISA. Microtiter plates coated with 8.0, 4.0, 2.0 or 1.0 μg/mL of rhCDK4 were incubated with serial dilutions of AK2 as part of the non-competitive ELISA assay. Affinity constants were calculated based on methods as described in the “Materials and Methods” sectionBack to article page