crRNA used | Enhancers1 | No. of pregnant rats/no. of treated rats | Total number of fetuses obtained | No. of fetuses or pups carrying pigmented eyes (successful KI [%]) | No. of fetuses showing indels (%) | No. of fetuses showing mosaic mutations (%) | No. of fetuses showing no mutation (%) |
---|---|---|---|---|---|---|---|
1 | – | 4/7 | 40 | 2 (5) | 16 (40) | 5 (13) | 23 (58) |
 | 1 µM SCR7 | 6/6 | 27 | 2 (7) | 11 (41) | 6 (22) | 16 (59) |
 | 10 µM SCR7 | 4/4 | 23 | 2 (9) | 10 (43) | 5 (22) | 13 (57) |
 | 100 µM SCR7 | 3/3 | 32 | 2 (6) | 16 (50) | 4 (13) | 15 (47) |
 | 4 µM EP Enhancer | 2/4 | 12 | 0 (0) | 4 (33) | 1 (8) | 8 (67) |
 | 30 µM AZT | 4/4 | 21 | 0 (0) | 10 (48) | 1 (5) | 11 (52) |
 | 5 µM L755 | 4/4 | 26 | 3 (12) | 9 (35) | 4 (15) | 17 (65) |
 | 15 µM L755 | 5/6 | 33 | 4 (12) | 14 (42) | 2 (6) | 17 (52) |
 | 15 µM RS-1 | 6/9 | 25 | 2 (8) | 12 (48) | 2 (8) | 13 (52) |
 | 30 µM HDR Enhancer | 4/4 | 28 | 3 (11) | 16 (57) | 8 (29) | 12 (43) |
 | Mixture of four enhancers2 | 3/3 | 21 | 2 (10) | 11 (52) | 3 (14) | 9 (43) |
2 | – | 4/4 | 21 | 5 (24) | 18 (86) | 2 (10) | 3 (14) |
 | 10 µM SCR7 | 3/3 | 30 | 5 (17) | 16 (53) | 4 (13) | 12 (40) |
 | 5 µM L755 | 3/4 | 18 | 3 (17) | 9 (50) | 2 (11) | 8 (44) |
 | 15 µM L755 | 3/3 | 20 | 6 (30) | 9 (45) | 3 (15) | 6 (30) |
 | 30 µM HDR Enhancer | 4/4 | 22 | 3 (14)2 | 10 (45) | 2 (9) | 11 (50) |
3 | – | 5/5 | 38 | 0 (0) | 7 (18) | 0 (0) | 31 (82) |
1 + 2 + 3 | – | 8/9 | 62 | 7 (11) | 25 (40) | 9 (15) | 35 (56) |