Table 2 Experimental design and results of the Box-Behnken Design
| Â | Factor 1 | Factor 2 | Factor 3 | Factor 4 | Enzyme activity (IU/mL) | |
|---|---|---|---|---|---|---|
Run | A: Glucose (g/L) | B: TPW (%) | C: MgSO4·7H2O (g/L) | D: CaCl2 (g/L) | Actual value | Predicted value |
1 | 22.5 | 100 | 1 | 1 | 7472.70 ± 137.28 | 7549.40 |
2 | 22.5 | 75 | 0.5 | 0.1 | 6863.40 ± 84.29 | 6484.19 |
3 | 22.5 | 100 | 0.5 | 0.55 | 7719.21 ± 69.39 | 7763.18 |
4 | 35 | 75 | 1 | 1 | 4637.23 ± 32.82 | 5219.90 |
5 | 10 | 75 | 0.5 | 0.55 | 5608.72 ± 118.44 | 5867.68 |
6 | 22.5 | 75 | 1 | 0.55 | 7202.28 ± 72.56 | 7045.27 |
7 | 22.5 | 50 | 1 | 1 | 2552.45 ± 40.53 | 2729.88 |
8 | 22.5 | 75 | 0.5 | 1 | 6631.05 ± 31.61 | 6298.53 |
9 | 22.5 | 100 | 1.5 | 0.55 | 7563.01 ± 50.01 | 7541.37 |
10 | 22.5 | 75 | 1 | 0.55 | 7306.88 ± 48.21 | 7045.27 |
11 | 22.5 | 50 | 1.5 | 0.55 | 2685.39 ± 156.66 | 2746.88 |
12 | 35 | 75 | 1.5 | 0.55 | 4927.65 ± 94.96 | 4905.82 |
13 | 22.5 | 75 | 1.5 | 1 | 6627.17 ± 75.44 | 6663.81 |
14 | 22.5 | 75 | 1 | 0.55 | 7163.78 ± 46.64 | 7045.27 |
15 | 35 | 100 | 1 | 0.55 | 6522.90 ± 55.96 | 5870.37 |
16 | 22.5 | 75 | 1 | 0.55 | 6900.12 ± 49.35 | 7045.27 |
17 | 10 | 75 | 1 | 1 | 5680.77 ± 71.76 | 5139.86 |
18 | 10 | 75 | 1.5 | 0.55 | 5507.49 ± 66.73 | 5462.89 |
19 | 10 | 50 | 1 | 0.55 | 1505.96 ± 69.34 | 1815.91 |
20 | 22.5 | 50 | 0.5 | 0.55 | 2586.87 ± 60.69 | 2713.96 |
21 | 10 | 100 | 1 | 0.55 | 6337.79 ± 52.37 | 6831.60 |
22 | 22.5 | 75 | 1 | 0.55 | 6653.31 ± 42.06 | 7045.27 |
23 | 35 | 50 | 1 | 0.55 | 1878.74 ± 55.13 | 1042.35 |
24 | 35 | 75 | 0.5 | 0.55 | 4408.22 ± 78.25 | 4689.94 |
25 | 22.5 | 75 | 1.5 | 0.1 | 5940.07 ± 78.91 | 5930.00 |
26 | 10 | 75 | 1 | 0.1 | 6290.44 ± 87.9 | 5813.23 |
27 | 22.5 | 50 | 1 | 0.1 | 2193.04 ± 137.11 | 2353.47 |
28 | 22.5 | 100 | 1 | 0.1 | 7317.97 ± 126.32 | 7377.67 |
29 | 35 | 75 | 1 | 0.1 | 3352.02 ± 175.91 | 3998.39 |