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Table 2 Conversions and product ee-values for the reduction of rac-2-phenylpropanal by isolated CtXR D51A.a,b

From: Reductive enzymatic dynamic kinetic resolution affording 115 g/L (S)-2-phenylpropanol

CtXR (U/mL)c

Phenylpropanol (µM)

ee (S)-Phenylpropanol

(%)

D51A (20)

76 ± 3

41 ± 4

D51A (3.4)

51 ± 5

98 ± 2

D51A (0.6)

13 ± 5

99.1 ± 0.3

  1. aNAD+ concentration 0.7 mM, reaction time 2 h. bThe phenylpropanol concentrations were measured by HPLC, the data represent the mean values of two reaction replicates and standard deviations from the mean. cThe enzyme activity (U/mL) was measured with 0.5 mM rac-2-phenylpropanal
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BMC Biotechnology

ISSN: 1472-6750

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