Fig. 5
Genotypes recovered in G1 BmGR66 knockout silkworms obtained by sib-mating of G0 mosaics. Mutations were detected in 17 G1 silkworms by PCR-amplification of regions surrounding the four sgRNA targeting sites. Amplified products were then subcloned and sequenced. Nucleotides shown in red are base substitutions; boldface represents insertions; dashes represent deletions. For large deletions or insertions, the sizes of the deleted or replaced regions are shown in parentheses