Fig. 4

Generation of BmBLOS2 knockout silkworms using GGN₂₀ format sgRNA and detection of germline transmission of mutations. A Left panel: G₀ BmBLOS2 mutant silkworm exhibiting mosaic translucent epidermal phenotype. Right panel: G₁ BmBLOS2 null mutant silkworm exhibiting completely translucent epidermal phenotype. Dazao was used as the wildtype control. Note that gender cannot be determined by visual examination at the developmental stage shown here (fifth instar larva). KO, knockout. B Genotypes of G₁ silkworms with detectable phenotypes. The wild type (WT) sgRNA target sequences are in blue and underlined, and PAM sequences are highlighted in red. In the mutants, dashes represent deleted nucleotides and the 4-nucleotide insertion is indicated using an open triangle