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Fig. 1 | BMC Biotechnology

Fig. 1

From: Expansion of targetable sites for the ribonucleoprotein-based CRISPR/Cas9 system in the silkworm Bombyx mori

Fig. 1

Genomic targets and corresponding sgRNAs in GGN20 or GGN18 formats. 20-nt genomic targets (not included the PAM) are shown in blue underlined text. The PAM is shown in red. The curved red line represents sequences that are common to all sgRNAs used in our experiments. Dotted lines represent mismatches between the sgRNA and genomic sequences. Note that GGN18 format sgRNAs were not generated for every target. A GGN18 format sgRNA for target BLOS2-T1. B GGN20 format sgRNA for target BLOS2-T1. C GGN20 format sgRNA for target GR66-T1 (no GGN18 format sgRNA). D GGN20 format sgRNA for target GR66-T2 (no GGN18 format sgRNA). E GGN20 format sgRNA for target GR66-T3 (no GGN18 format sgRNA). F GGN20 format sgRNA for target GR66-T4 (no GGN18 format sgRNA). G GGN18 format sgRNA for target GR66-T5. H GGN20 format sgRNA for target GR66-T5. Only one mismatch occurs between the 5′ end of this sgRNA and its genomic target

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