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Fig. 4 | BMC Biotechnology

Fig. 4

From: Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor

Fig. 4

Effect of wild-type and mutant recombinant equine chorionic gonadotropin (rec-eCG) on cyclic adenine monophosphate (cAMP) production in cells expressing rat lutropin/chorionic gonadotropin receptor (rLH/CGR). Cells transiently transfected with rLH/CGR were seeded in 384-well plates (10,000 cells per well) at 24 h post-transfection. The cells were incubated with re-eCG proteins for 30 min at room temperature. cAMP production was detected using a homogeneous time-resolved fluorescence assay and was represented as Delta F%. The cAMP concentrations were calculated using GraphPad Prism software. The results of the mock-transfected cells were subtracted from each dataset (see Methods). Each data point represents mean ± standard error of mean from triplicate experiments. The mean data were fitted to the equation of a one-phase exponential decay curve. The blank circles show the data of the wild-type receptor

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BMC Biotechnology

ISSN: 1472-6750

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