Extraction and washing condition | RNA concentration(ng/µL) Median (range) | Detected phenol contaminant at 260 nm Median (range) |
---|---|---|
Once chloroform extraction without EtOH washing | 294.0 (240.7–381.45) | 6.44 (5.15–8.23) |
Once chloroform extraction and EtOH washing | 152.4 (150.0–175.9) | 3.43 (2.13–4.00) |
Ttriple chloroform extraction without EtOH washing | 201.3 (158.0–215.0) | 4.00 (2.94–4.08) |
Triple chloroform extraction and EtOH washing | 130.2 (102.8–158.1) | 2.63 (2.07–2.95) |