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Table 3 Effect of multiple chloroform extraction and ethanol washing on elimination of RNA yield and phenol (n = 3)

From: Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency

Extraction and washing condition RNA concentration(ng/µL) Median (range) Detected phenol contaminant at 260 nm Median (range)
Once chloroform extraction without EtOH washing 294.0 (240.7–381.45) 6.44 (5.15–8.23)
Once chloroform extraction and EtOH washing 152.4 (150.0–175.9) 3.43 (2.13–4.00)
Ttriple chloroform extraction without EtOH washing 201.3 (158.0–215.0) 4.00 (2.94–4.08)
Triple chloroform extraction and EtOH washing 130.2 (102.8–158.1) 2.63 (2.07–2.95)