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Table 2 Comparison of slop, amplification efficiency, and R2 estimated through the linear regression of triple standard curves of Cel-miRNA-39-1 according to the annealing temperature

From: Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency

Parameters Annealing temp. (℃)
58 60 62
Slope
 Median − 4.144 − 3.554 − 3.648
 Range − 4.5042 to − 3.8800 − 3.5939 to − 3.3490 − 4.1735 to − 3.6066
 95% CI − 4.953 to − 3.396 − 3.825 to − 3.173 − 4.594 to − 3.024
Efficiency (%)
 Median 74.40 91.15 62.01
 Range 66.7 to 81.0 89.8 to 98.9 73.6 to 89.3
 95% CI 56.29 to 91.81 81.08 to 105.5 62.01 to 105.3
R2
 Median 0.9970 0.9993 0.9962
 Range 0.9820 to 0.9993 0.9907 to 0.9999 0.9899 to 0.9973
 95% CI 0.9696 to 1.016 0.9838 to 1.009 0.9845 to 1.004