Fig. 2From: Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiencyStandard curves of synthetic Cel-miRNA-39-1 according to annealing temperature. The Cq values were plotted against log of four different ten-fold cDNA dilutions (1, 10, 100, and 1000) of generated from synthetic Cel-miRNA-39-1. This series of four dilutions was measured in three replicates per dilutionBack to article page