Fig. 1From: Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiencyStandard curves of synthetic miRNA-16 according to annealing temperature. The Cq values were plotted against log of four different ten-fold cDNA dilutions (1, 10, 100, and 1000) generated from synthetic miRNA-16. This series of four dilutions was measured in three replicates per dilutionBack to article page