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Table 1 the effect of protease inhibitors on the activity of NK-Bs

From: Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity

inhibitor

residual activity (%)

1 mM

2 mM

β-mercaptoethanol

97.48 ± 3.72

92.90 ± 3.51

EDTA

109.46 ± 2.06

105.11 ± 4.50

PMSF

40.17 ± 1.84

3.02 ± 0.36

control

100.00 ± 1.62

100.00 ± 1.27