From: Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity
inhibitor
residual activity (%)
1 mM
2 mM
β-mercaptoethanol
97.48 ± 3.72
92.90 ± 3.51
EDTA
109.46 ± 2.06
105.11 ± 4.50
PMSF
40.17 ± 1.84
3.02 ± 0.36
control
100.00 ± 1.62
100.00 ± 1.27