Fig. 5From: Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activityPurification of NK-Bs. A SDS-PAGE of purified NK-Bs. M: protein molecular weight marker (the molecular weight of each band is indicated on the left). Lane 1: supernatant from shake-flask fermentation. Lane 2: target protein purified with Ni2+-affinity chromatography. B Glycosylation analysis of NK-Bs. Lane 1: purified NK-Bs. Lane 2: NK-Bs treated with Endo HBack to article page