Skip to main content
Fig. 2 | BMC Biotechnology

Fig. 2

From: Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity

Fig. 2

Correlation between recombinant protein titer and gene copy number. A The relationship between NK-Bs expression levels and aprN gene copy number were assessed using a plate assay. The copy number of the target gene in each strain is indicated below each colony. The circle around each colony represents the average halo size of responding multicopy expression strains. Scale bar, 1 cm. B SDS-PAGE analysis of NK-Bs secreted into the culture supernatant. M: protein molecular weight marker (the molecular weight of each band is indicated on the left); lanes 1–7: supernatants from shake-flask fermentation of NK-Bs 1–7 strains, respectively, collected after an 84-h induction with methanol. A total of 20 μL of each sample was loaded

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us