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Fig. 3 | BMC Biotechnology

Fig. 3

From: Analysis of conventional and alternative CRISPR/Cas9 genome editing to enhance a single-base pair knock-in mutation

Fig. 3

Editing using a neomycin cassette coupled homologous recommendation results in highly specific heterozygous mutations. A Schematic presentation of left homology arm (LHA), right homology arm (RHA), and gRNA in respect to location of T55 residue (red box). B, C Percentage of specific mutations and chromatogram confirming heterozygous T55A mutation. Red box indicates single base pair target. Note data represents sequence data of template strand

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BMC Biotechnology

ISSN: 1472-6750

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