Skip to main content
Fig. 2 | BMC Biotechnology

Fig. 2

From: Analysis of conventional and alternative CRISPR/Cas9 genome editing to enhance a single-base pair knock-in mutation

Fig. 2

Ribonucleoprotein (RNP) complex, the proximity of the cut to the edit site, and inclusion of a silent mutation increase efficiency of single base pair edits. A Design of gRNA (green), PAM (orange), silent mutation (purple box) in respect to target T55 location (red box). B, C Locations and types of mutations in the first round of RNP (RNP 1, green) and the second round of RNP (RNP 2, yellow) of editing. Scissors indicate Cas9 cut site. D Chromatogram of Wildtype (top), heterozygous (middle), and homozygous (bottom) T55A mutations. Note data represents sequence data of template strand

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us