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Fig. 1 | BMC Biotechnology

Fig. 1

From: Analysis of conventional and alternative CRISPR/Cas9 genome editing to enhance a single-base pair knock-in mutation

Fig. 1

Traditional plasmid-based CRISPR/Cas9 genome editing results in high incidence of indel mutations. A Design of gRNA (green) and PAM sequence (orange) in respect to location of T55 residue (red box). B, C Percentage of specific mutations (B) and their location (C) from the target edit site (designated as 0}. Red indicates target edit and scissors indicate Cas9 cut site. Note data represents sequence data of template strand

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BMC Biotechnology

ISSN: 1472-6750

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