Fig. 6
From: Evaluation of RNA isolation methods for microRNA quantification in a range of clinical biofluids
Colorimetric effects on RT-qPCR results in bile. (A) Visual observation of total RNA isolated from ten bile samples using the miRCURY RNA isolation kit – biofluids showed four samples had greenish discoloration. RNA samples isolated by other methods did not show any coloration (not shown) (B) The coloration of RNA samples correlated with low detection levels of cel-miR-39 (upper panel) and cel-miR-54 (lower panel). Black circles represent non-colored samples, green circles represent the greenish discolored samples. The unreliable detection area is indicated in grey. Detection levels are calculated from Cq values using the following equation: detection levels = 2(−Cq)× 109. RN; Qiagen miRNeasy kit, QP; Qiazol in combination with dr. GenTLE precipitation carrier, NG; NORGEN Total RNA isolation kit, CU; miRCURY RNA isolation kit - biofluids