Fig. 5
From: Evaluation of RNA isolation methods for microRNA quantification in a range of clinical biofluids
Detection levels of spiked-in cel-miR-54 in serum,urine, perfusate and bile. Levels of cel-miR-54, spiked during cDNA synthesis, are determined in serum (A), urine (B), perfusate (C), and bile (D) from six liver patients and their donor organs. All samples were treated with Heparinase I, and no statistically significant differences were observed between groups. Red lines indicate the detection levels of the reference sample (cel-miR-54 spiked in RNA-free water). RN; Qiagen miRNeasy kit, QP; Qiazol in combination with the dr. GenTLE precipitation carrier, NG; NORGEN Total RNA isolation kit, and CU; miRCURY RNA isolation kit – biofluids. Detection levels are calculated from Cq values using the following equation: detection levels = 2(−Cq)× 109