Fig. 3
From: Evaluation of RNA isolation methods for microRNA quantification in a range of clinical biofluids
Heparinase I treatment improves quantification levels in biofluids with high heparin contamination. Total RNA from four different graft perfusate samples known to contain heparin were co-incubated with (+) or without (−) 6 IU heparinase I during cDNA synthesis. Results are shown for spike in miRNAs cel-miR-54 (A), cel-miR-39 (B) and endogenous miRNAs, miR-122 (C) and miR-222 (D) for three different RNA isolated methods, RN; Qiagen miRNeasy kit, QP; Qiazol in combination with the dr. GenTLE precipitation carrier, NG; NORGEN Total RNA isolation kit. Notably the NG method is the least sensitive for heparin co-purification and showed the smallest differences in detection levels between with or without heparinase I. Detection levels are calculated from Cq values using the following equation: detection levels = 2(−Cq)× 109