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Table 2 Kinetic parameters of phenolic acid substrates of P-WT and mutants

From: Improving the catalytic characteristics of phenolic acid decarboxylase from Bacillus amyloliquefaciens by the engineering of N-terminus and C-terminus

   Kinetic constanta
P-Coumaric acid Ferulic acid Caffeic acid Sinapic acid
P-C Vmax (IU/mg) 193.80 ± 2.38 97.73 ± 5.86 65.66 ± 4.79 12.36 ± 0.79
Km (mmol/L) 2.05 ± 0.29 1.03 ± 0.38 3.04 ± 0.69 1.31 ± 0.03
kcat/Km (mmol/L/s) 13.41 ± 0.45 28.15 ± 0.28 23.24 ± 0.52 40.87 ± 0.28
P-N Vmax (IU/mg) 683.10 ± 7.38 208.50 ± 8.38 143.80 ± 5.89 10.36 ± 0.34
Km (mmol/L) 7.02 ± 0.61 2.14 ± 0.83 1.48 ± 0.73 1.06 ± 0.42
kcat/Km (mmol/L/s) 14.53 ± 0.53 33.57 ± 0.49 37.57 ± 0.62 28.44 ± 0.53
P-m1 Vmax (IU/mg) 17.26 ± 0.38 19.07 ± 8.39 124.60 ± 3.86 16.66 ± 0.78
Km (mmol/L) 1.88 ± 0.53 2.08 ± 0.28 1.36 ± 0.28 1.82 ± 0.38
Kcat/Km (mmol/L/s) 152.38 ± 1.12 28.75 ± 0.39 32.14 ± 0.52 19.17 ± 0.19
P-m2 Vmax (IU/mg) 136.20 ± 8.36 111.90 ± 10.91 72.64 ± 6.89 5.08 ± 0.58
Km (mmol/L) 1.22 ± 0.26 1.00 ± 0.38 6.49 ± 0.53 4.55 ± 0.61
kcat/Km (mmol/L/s) 15.97 ± 0.27 36.54 ± 0.36 4.07 ± 0.64 13.24 ± 0.66
P-Nm1 Vmax (IU/mg) 254.40 ± 7.38 103.20 ± 7.48 87.37 ± 8.64 5.60 ± 0.53
Km (mmol/L) 3.52 ± 0.32 1.43 ± 0.06 1.21 ± 0.43 0.77 ± 0.64
kcat/Km (mmol/L/s) 11.31 ± 0.36 19.94 ± 0.73 24.72 ± 0.63 45.67 ± 0.25
P-Nm2 Vmax (IU/mg) 186.30 ± 6.39 99.09 ± 0.3.83 144.20 ± 7.32 5.27 ± 0.17
Km (mmol/L) 12.05 ± 0.27 6.4 ± 0.38 9.33 ± 0.28 3.41 ± 0.26
kcat/Km (mmol/L/s) 10.33 ± 0.18 14.14 ± 0.59 10.38 ± 0.97 11.69 ± 0.11
P-WT Vmax (IU/mg) 121.50 ± 7.88 22.32 ± 4.31 89.46 ± 10.35 9.22 ± 0.19
Km (mmol/L) 4.09 ± 0.59 2.39 ± 0.58 3.10 ± 0.47 3.20 ± 0.30
kcat/Km (mmol/L/s) 9.74 ± 0.53 16.49 ± 0.42 9.76 ± 0.26 16.94 ± 0.58
  1. a These values were calculated by taking the average of three experiments conducted using SD