Fig. 4From: Lentiviral CRISPR-guided RNA library screening identified Adam17 as an upstream negative regulator of Procr in mammary epitheliumKnockdown of ADAM17 increases PROCR level in TNBC cell lines. a Validation of ADAM17 shRNAs knockdown efficiency in MDA-MB-231 cells by RT-qPCR. b RT-qPCR results showing PROCR mRNA remain unchanged in MDA-MB-231 cells after knockdown of ADAM17. c Knockdown of ADAM17 in MDA-MB-231 cells resulted in increased protein levels of membrane expressing PROCR. d Validation of ADAM17 shRNAs knockdown efficiency in Hs578T cells by RT-qPCR. e RT-qPCR results showing PROCR mRNA remain unchanged in Hs578T cells after knockdown of ADAM17. f Knockdown of ADAM17 in Hs578T cells resulted in increased protein levels of membrane expressing PROCR. a-b, d-e, Data are presented as mean ± S.E.M. n.s, not significant. ****P < 0.0001Back to article page