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Table 5 Contents of F1culture medium

From: Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Materials Glycerol KH2pO4 K 2HpO4 (NH4)2SO4 Yeast Extract Peptone MgSo4 Trace element
Volume (g/l) 20-40 9 6 0.5 5 5 1 1 (ml/l)