From: Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide
Materials
Glycerol
KH2pO4
K 2HpO4
(NH4)2SO4
Yeast Extract
Peptone
MgSo4
Trace element
Volume (g/l)
20-40
9
6
0.5
5
1
1 (ml/l)