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Table 4 Analysis of variance (ANOVA) of the response surface model for the cytoplasmic hGH hormone production

From: Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Source

Sum of Squares

df

Mean Square

F-Value

P-value

Prob>F

Model

5905.05

10

5905.05

4.92

0.0095

Significant

A

73.12

1

73.12

0.61

0.4532

 

B

450.00

1

450.00

3.75

0.0816

 

C

864.90

1

864.90

7.21

0.0229

 

D

1058.00

1

1058.00

8.81

0.0141

 

AB

360.00

1

360.00

3.00

0.1140

 

AC

72.00

1

72.00

0.60

0.4566

 

AD

490.00

1

490.00

4.08

0.0709

 

BC

1300.50

1

1300.50

10.83

0.0081

 

BD

24.04

1

24.04

0.20

0.6641

 

CD

1104.50

1

1104.50

9.20

0.0126

 

Residual

1200.29

10

120.03

   

Lack of fit

619.09

6

103.18

0.71

0.6635

Not Significant

Pure Error

581.20

4

145.30

   

Cor Total

7105.81

20

    

Std.Dev.

Mean

R-Squared

    

10.96

26.90

0.83

    
  1. A: IPTG (mM), B: Temperature (ËšC), C: Cell density at induction time (OD600), D: Post induction time (h)