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Table 4 Analysis of variance (ANOVA) of the response surface model for the cytoplasmic hGH hormone production

From: Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Source Sum of Squares df Mean Square F-Value P-value Prob>F
Model 5905.05 10 5905.05 4.92 0.0095 Significant
A 73.12 1 73.12 0.61 0.4532  
B 450.00 1 450.00 3.75 0.0816  
C 864.90 1 864.90 7.21 0.0229  
D 1058.00 1 1058.00 8.81 0.0141  
AB 360.00 1 360.00 3.00 0.1140  
AC 72.00 1 72.00 0.60 0.4566  
AD 490.00 1 490.00 4.08 0.0709  
BC 1300.50 1 1300.50 10.83 0.0081  
BD 24.04 1 24.04 0.20 0.6641  
CD 1104.50 1 1104.50 9.20 0.0126  
Residual 1200.29 10 120.03    
Lack of fit 619.09 6 103.18 0.71 0.6635 Not Significant
Pure Error 581.20 4 145.30    
Cor Total 7105.81 20     
Std.Dev. Mean R-Squared     
10.96 26.90 0.83     
  1. A: IPTG (mM), B: Temperature (˚C), C: Cell density at induction time (OD600), D: Post induction time (h)