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Table 3 Analysis of variance (ANOVA) of the response surface model for the periplasmic hGH hormone production

From: Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Source

Sum of Squares

df

Mean Square

F Value

P-value

Prob>F

Model

263.58

10

26.36

3.23

0.0393

Significant

A

23.93

1

23.93

2.93

0.1178

 

B

6.55

1

6.55

0.80

0.3918

 

C

78.11

1

78.11

9.56

0.0114

 

D

42.00

1

42.00

5.14

0.0468

 

AB

16.74

1

16.74

2.05

0.1828

 

AC

25.43

1

25.43

3.11

0.1082

 

AD

1.129

1

1.129

1.39

0.9909

 

BC

12.47

1

12.47

1.53

0.2451

 

BD

3.78

1

3.78

0.46

0.5119

 

CD

22.67

1

22.67

2.72

0.1268

 

Residual

81.73

10

8.17

   

Lack of fit

55.64

6

9.27

1.42

0.3824

Not Significant

Pure Error

26.09

4

6.52

   

Cor Total

345.31

20

    

Std.Dev.

Mean

R-Squared

    

2.86

5.80

0.76

    
  1. A: IPTG (mM), B: Temperature (ËšC), C: Cell density at induction time (OD600), D: Post induction time (h)