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Table 3 Analysis of variance (ANOVA) of the response surface model for the periplasmic hGH hormone production

From: Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Source Sum of Squares df Mean Square F Value P-value Prob>F
Model 263.58 10 26.36 3.23 0.0393 Significant
A 23.93 1 23.93 2.93 0.1178  
B 6.55 1 6.55 0.80 0.3918  
C 78.11 1 78.11 9.56 0.0114  
D 42.00 1 42.00 5.14 0.0468  
AB 16.74 1 16.74 2.05 0.1828  
AC 25.43 1 25.43 3.11 0.1082  
AD 1.129 1 1.129 1.39 0.9909  
BC 12.47 1 12.47 1.53 0.2451  
BD 3.78 1 3.78 0.46 0.5119  
CD 22.67 1 22.67 2.72 0.1268  
Residual 81.73 10 8.17    
Lack of fit 55.64 6 9.27 1.42 0.3824 Not Significant
Pure Error 26.09 4 6.52    
Cor Total 345.31 20     
Std.Dev. Mean R-Squared     
2.86 5.80 0.76     
  1. A: IPTG (mM), B: Temperature (˚C), C: Cell density at induction time (OD600), D: Post induction time (h)